In: Biology
1. How do the three objective lenses on the microscope you used differ and what are the advantages and disadvantages of each?
2. How variable were the lengths of Paramecium caudatum cells relative to the lengths and widths of the cells you observed in Elodea leaves?
3. What size range did the single eukaryotic cells you examined span?
4. What subcellular features did you recognize and identify in all of the organisms you examined
5. Did you examine any prokaryotes in the pond or aquarium water you used?
6. How would prokaryotes and eukaryotes differ when viewed with a microscope?
7.What organisms were you able to identify in the pond or aquarium water you examined and how did they differ?
1. The three objectives lenses present in microscope are 10X, 40X and 100X. 10X is the eyepiece lense. 40X is the total magnification and 100X is the oil immersion with highest magnification and resolution among these three. The big advantage in the field of optical trapping mainly due to the not so strict trapping spatial conditions.The only disadvantage with normal microscopes are resolution of the image we get is not sharp and crisp. Also the range is limited.
2. Elodea cells have cell walls, nuclei, a central vacuole and chloroplasts visible through the light microscope. Using 40X objective length and variable size of Paramecium caudatum can be detected. The Paramecium caudatum cells have lengths of about 150µm than the cells found in the Elodea leaves (140µm length and 75µm width).
3. Range of sizes;
Paramecium caudatum - 116-175µm in length
Elodea leaves - 105-175µm (length range) & 70-90µm (width range)
4. There is a cell wall in common. And all eukaryotic cell organelles present are common in the organisms examined.
5. Ample number of variety of microorganisms were found in the pond or aquarium water.
6. The major difference between the prokaryotes and eukaryotes when viewed under microscope is, presence of nuclear membrane in eukaryotes and absence of nuclear membrane in prokaryotes.
7. Three organisms found within the pond water included algae, leeches, and Stentor. Algae is photosynthetic (green colored) with many complex shapes. Leeches are snake like organisms. Stentor is trumpet-shaped organism with dark pigmentation. They consume the food material from their vicinity with the help of axilliary structures (cilia in case of stentor).