Question 1 Create an image of one cycle of the PCR process. Draw a diagram of...

Question 1

Create an image of one cycle of the PCR process. Draw a diagram of this process as shown in class (one color for template, another color for DNA synthesized

Which gene are we amplifying (making copies of) in whatever experiment? Why could we choose this gene?

Expert Solution

Answer -aa-

b-

The PCR process was originally developed to amplify short segments of a longer DNA molecule (Saiki et al. 1985). A typical amplification reaction includes target DNA, a thermostable DNA polymerase, two oligonucleotide primers, deoxynucleotide triphosphates (dNTPs), reaction buffer and magnesium.

Polymerase chain reaction (PCR) is a method widely used in molecular biology to make many copies of a specific DNA segment. Using PCR, a single copy (or more) of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.

Using PCR, a DNA sequence can be amplified millions or billions of times, producing enough DNA copies to be analyzed using other techniques. ... For instance, PCR is used to amplify genes associated with genetic disorders from the DNA of patients (or from fetal DNA, in the case of prenatal testing).

Nightingale answered 5 months ago

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