Question

A. Describe three important differences in the transcription of mRNA's between bacterial and eukaryotic cells.

B. In the synthesis of cDNA libraries for eukaryotic genes, reverse transcriptase can be used with a primer containing poly(T). Why is this NOT useful for prokaryotic systems?

Answer 1

A. Difference between transcription of mRNA's between bacterial and eukaryotic cells.

Eukaryotic transcription	Prokaryotic Transcription
Have three RNA polymerase I, II, amd III	Have one RNA polymerase
the RNA is first transcribed in the nucleus and then translated in the cytoplasm.	transcription and translation occurs simultaneously in cytoplasm
Requires proteins known as transcription factors for the initiation.	Doesn’t require any proteins or other factors for the initiation of transcription

B. In prokaryotes very less RNAs are poly adenylated as it leads to degradation and as the transcrition and translation occurs simultaneously in cytosol. In eukaryotes all coding mRNAs will have poly A tail for protection during tranfer from nucleus to cytosol.  As  there is no poly A tail in all prokaryotic mRNA we acnt use Poly T primer for reverse transcrition in prokaryotic system.