Question

Decribe the process of specification of paraxial mesoderm.

please include the role of Noggin, Fox1/Fox2, Tbx6, mesogenin1, retinoic acid, and Fgf8.

Answer 1

Paraxial mesoderm, also known as presomiticor somitic mesoderm is the area of mesoderm in the neurulating embryo that flanks and forms simultaneously with the neural tube. The cells of this region give rise to somites, blocks of tissue running along both sides of the neural tube, which form muscle and the tissues of the back, including connective tissue and the dermis.

The paraxial and other regions of the mesoderm are thought to be specified by bone morphogenetic proteins, or BMPs, along an axis spanning from the center to the sides of the body. Members of the FGF family also play an important role, as does the WNTpathway. In particular, Noggin, a downstream target of the Wnt pathway, antagonizes BMP signaling, forming boundaries where antagonists meet and limiting this signaling to a particular region of the mesoderm. Together, these pathways provide the initial specification of the paraxial mesoderm and maintain this identity.This specification process has now been fully recapitulated in vitro with the formation of paraxial mesoderm progenitors from pluripotent stem cells, using a directed differentiation approach.

The tissue undergoes convergent extension as the primitive streak regresses, or as the embryo gastrulates. The notochord extends from the base of the head to the tail; with it extend thick bands of paraxial mesoderm.[3]

As the primitive streak continues to regress, somites form from the paraxial mesoderm by "budding off" rostrally.

In certain model systems, it has been shown that the daughter cells of stem cell-like progenitor cells which come from the primitive streak or site of gastrulation migrate out and localize in the posterior paraxial mesoderm. As the primitive streak regresses and somites bud off anteriorly, new cells derived from these stem-cell like precursors constantly enter the posterior end of the paraxial mesoderm.

Many kinds of tissue derive from the segmented paraxial mesoderm by means of the somite. Among these are:

• the sclerotome, which forms cartilage,
• the syndetome, which forms tendons,
• the myotome, which forms skeletal muscle,
• the dermatome, which forms the dermis as well as skeletal muscle,
• and endothelial cells.

Head mesodermEdit

A particular kind of tissue deriving from the paraxial mesoderm is the head mesoderm. This tissue derives from the unsegmented paraxial mesoderm and prechordal mesoderm. Tissues derived from the head mesoderm include connective tissue and the muscles of the face.

The head mesoderm forms through a separate signaling circuit than the segmented paraxial mesoderm, though also involving BMP and fibroblast growth factor (FGF) signaling. Here, retinoic acid interacts with these pathways.