Question

Compare and contrast sterilization and sanitization; give a detailed descriptions of ALL techniques discussed in the text and notes used for both.

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Answer 1

There is huge difference in sanitization and sterilization process. one has ie sanitization is cleaning a thing or living /cell from infecting bacteria/virus/other harmful conditions, while sterilization is completely removing off any kind of living cells/infecting things , even completely cut off from the living environment.

Obviously the techniques for both are also different or different in a level.
sanitization techniques are

1. cleaning by water- simplest

2. cleaning by soaps and detergents- it should be of good properties.

3.High Pressure Washing - for walls, floors,Spray can form aerosols mist from irritating chemicals

4.Foam Cleaning - for small sections

sterilization techniques are

!. wet heat- AUTOCLAVING ,using pressurised steam to heat the material to be sterilised. very effective

2. dry heat- FLAMING/BAKING, dry heat tends to kill microbes by oxidation of cellular components.Not for protein hydrolysis.

3.filtration - passing the solution through a filter with a pore diameter that is too small for microbes to pass through.its a quick way.

4. solvents-Ethanol, Isopropanol are solvents. denaturing proteins through a process that requires water, so they must be diluted to 60-90% in water to be effective.

5. radition-UV, x-rays and gamma rays they damage DNA there by killing the cells completely.