Question

Suppose that a scientist was conducting an experiment and decided to transplant GFP-labeled cells from the dorsal region of a frog embryo at the 120-cell embryonic stage into the putative ventral side of an unlabeled zebrafish embryo of an equivalent developmental stage, state one question the scientist might have been trying to answer. Explain how this experimental design would answer the question you stated.

Transplantation could be used to build a fate map of an early embryo.

True or False

Answer 1

State one question the scientist might have been trying to answer.

The experiment purpose was to map the embryonic origin of the tissue of interest in the adult zebrafish (fate mapping). An embryo reaches the blastocyst stage at nearly 120 cells. Cells in a blastocyst are pluripotent (stem cells), which means they have the capacity to create any type of cell. In this experiment, the embryonic cells from the frog will adopt the fate map of host tissue, as they are being transplanted before any differentiation of the cells.

Explain how this experimental design would answer the question you stated.

As the donated cells are marked with a fluorescent agent, the scientist will be able to locate them in the adult zebrafish and see if the cell in which they differentiated and its location

Transplantation could be used to build a fate map of an early embryo. TRUE

A tissue from donor embryo is excised and transplanted to the host tissue, the behavior of donor tissue in new circumstances help in tracing the embryological development pathway. If the transplantation is done before the specification of the tissue, it will adopt the fate map of host tissue, but if transplantation is done after specification, donor tissue will follow its own fate inside the host.