Question

What are the effects of different concentration of fish oil on CD56, CD3, CD19, CD4, CD8, HLA-DR in a flow cytometry experiment? What would you expect the flow cytometry of these markers to be (explain in details by grouping markers like 56-3 etc...)

Answer 1

The effects of fish oil which contains different amounts of the fatty acids on the cluster of differentiation (CD) receptors present on the T cells and B cells as given below in the table:

The effects of the olive oil, docosahexaenoic acid and fish oil on the CD3, CD4 and CD 8 receptor on the T cells is shown. The Fish oil reduced the levels of the circulating B and T cells in the blood.

1) The levels of the receptors were the same in the CD4/CD3 positive T cells before and after treatment with fish oil as compared to the levels of CD45 receptor.

2)The level of the CD8/CD3 T cytotoxic cells increased after fish oil and polyunsaturated fatty acid treatment. The intensity of increase was more in fish oil treated samples.

3)The levels of CD 19 as compared to the CD 45 levels were the same in the fish oil treated samples in the B cells.

4) The levels of the CD 14 monocytes decreased as compared to the CD 45 levels when both fish oil and DHA was added to the sample (docosahexaenoic acid).

5) Fish oil decreases the accumulation and aggregation of the MHC-I and MHC-II molecules on the receptor after addition of the fish oil and cyclodextrins in the JY cell lines. The levels of CD 3 receptor expression in the T cells was less as compared to the expression of other receptors in the samples. The recruitment of PKC during the receptor signaling is decreased in the B-T cell signaling as given in the diagram below: