Is EcoR1 a 5’ and 3’ overlap?

Expert Solution

Yes

The overhangs produce after digestion of DNA with EcoR1 are complementary to each other due to presence of overhang and therefore can base pair again to form intact fragments when the restriction enzyme is removed. These ends which can pair up again are called as sticky ends.

Restriction enzymes are the enzymes which recognise a palindrome its sides on the DNA and leave it at a specific location. After cutting, they can either produce sticky and like EcoR1 or blunt ends which do not overlap due to absence of overhang like Sma1.

gladiator answered 2 years ago

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