Question

What are three main differences between High Performance Liquid Chromatography and Gas Chromatography?

Answer 1

Differences between High performance liquid chromatography and Gas Chromatography:

1.The major difference between GC and HPLC lies in the phases used. While in GC, compounds of a mixture are seperated using a liquid (stationary) phase and a gas (mobile) phase, in case of HPLC the stationary phase is a solid while liquids make up mobile phase.

2. Another difference lies in temperature control during the process. In GC, there is an oven to contain the coloumn consinsting of gas phase and it can control the temperatures when the gases are passing through the column. On the other hand, there is not such provision of temperature control in HPLC.

3. The last difference pertains to the concentration of the compounds. In GC, it is the vapour pressure of the gases that decide the concentration of compounds whereas it is possible to increase or decrease the concentration of the compounds in HPLC.