Genetics Essay 1. Describe DNA vector cloning. In your description, make sure to include: a) the...

Genetics Essay

1. Describe DNA vector cloning. In your description, make sure to include:

a) the process (steps)

b) three things that a plasmid cloning vector must have

c) an explanation of how blue and white screening works during DNA recombinant technology

Expert Solution

(a) In the process of DNA vector cloning: small fragment of foreign DNA is joined into a plasmid by cutting both the DNA with Restriction Endonuclease forming a recombinant DNA (rDNA). The rDNA is then transfer to the host cell (E. coli) where it stably inherited and synthesis the recombinant product.

(b) The three things that plasmid cloning vector must have are: origin of replication, selectable marker and multiple cloning site.

(c) blue white screening of products during recombinant DNA technology is: it is based on the principle of Insertional inactivation.

The details explanation of (a) (b) & (c) is given in the images. Please go through the images

for further queries please do comment, thank you ?

gladiator answered 2 years ago

Describe the structure of the DNA molecule. In your description, be sure to include the names...

Describe the structure of the DNA molecule. In your description, be sure to include the names of the components that comprise the molecule, as well as the complementary base pairing rules.

1.) Describe the four stages of genetic engineering: cleave DNA & insert into cloning vector -producing...

1.) Describe the four stages of genetic engineering: cleave DNA & insert into cloning vector -producing recombinant DNA, insert recombinant vector into bacterial cells -cloning, amplification of recombinant vector, screening for recombinant DNA. 2.) What and how are antibiotic resistance and the lacZx gene used in screening? 3.) What is Agrobacterium tumefaciens? What normally occurs upon Agrobacterium tumafaciencs infection in plants? How is it used to genetically engineer plants (stably insert foreign genes into plants)?

describe recombinant DNA cloning,

Experiment 2- Cloning a DNA Fragment to a Bacterially-Derived Plasmid Vector “Table 1: Fragment Lengths” “DNA...

Experiment 2- Cloning a DNA Fragment to a Bacterially-Derived Plasmid Vector “Table 1: Fragment Lengths” “DNA Type” “Longest Length (in base pairs)” “Foreign” 720 “Plasmid” 2804 1. What is the expected size of the plasmid plus the cut foreign DNA? 2. What type of ends to the enzymes BamHI and EcoRI produce? How does this type of end facilitate cloning? 3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA...

Cloning a DNA Fragment to a Bacterially-Derived Plasmid Vector” “Table 1: Fragment Lengths” DNA TYPE: LONGEST...

Cloning a DNA Fragment to a Bacterially-Derived Plasmid Vector” “Table 1: Fragment Lengths” DNA TYPE: LONGEST LENGTH FOREIGN: 716 PLASMID: 2837 Post-Lab Questions 1. What is the expected size of the plasmid plus the cut foreign DNA? 2. What type of ends to the enzymes BamHI and EcoRI produce? How does this type of end facilitate cloning? 3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA molecule? How does...

Describe in detail the action of insulin. Be sure to include a description of what it...

Describe in detail the action of insulin. Be sure to include a description of what it is (what kind of biomolecule/class of hormone), how its secretion is regulated, where specifically it is secreted from, its general effects on body cells, and finally each of its specific effects (if it has any) on: skeletal muscle, the liver, adipose tissue, and blood glucose levels.

Experiment 3: Cloning a DNA Fragment into a Bacterially-Derived Plasmid Vector Foregin DNA = 720 Plasmid...

Experiment 3: Cloning a DNA Fragment into a Bacterially-Derived Plasmid Vector Foregin DNA = 720 Plasmid DNA 2804 Post-Lab Questions 1. What is the expected size of the plasmid plus the cut foreign DNA? 2524 base pairs 2. What type of ends do the enzymes BamHI and EcoRI produce? How does this type of end facilitate cloning? 3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA molecule? How does...

Discuss the limitation of DNA polymerase. Make sure to include the consequence of these limitations, especially...

Discuss the limitation of DNA polymerase. Make sure to include the consequence of these limitations, especially for eukaryotic chromosomes, and how these organisms have evolved to counter these limitations.

Make sure to include comments that explain all your steps (starts with #) Make sure to...

Make sure to include comments that explain all your steps (starts with #) Make sure to include comments that explain all your steps (starts with #) Write a program that prompts the user for a string (a sentence, a word list, single words etc.), counts the number of times each word appears and outputs the total word count and unique word count in a sorted order from high to low. The program should: Display a message stating its goal Prompt...

List the five DNA condensation levels starting from the DNA double helix. Make sure to describe...

List the five DNA condensation levels starting from the DNA double helix. Make sure to describe what the structure looks like, the size of each structure, and what proteins, if any, are involved.

Question