You have sequenced the genomes of males of two fruitfly strains. When comparing the sequences of...

You have sequenced the genomes of males of two fruitfly strains. When comparing the sequences of genes, you found that strain #1 has a gene that’s not present in strain #2. What would you need to do to identify the function of this novel gene?

		randomly mutagenize the fly embryos of both strains
		use RNAi to silence the gene in strain #1 and observe the phenotype
		fuse the gene in strain #1 with GFP and observe the embryo
		perform a forward genetic screen in both strains

Expert Solution

The gene here is present in strain 1 but it is absent in strain 2. We are to determine the function of the gene. To determine the function, a simple method is to stop the gene from functioning and see as to what phenotype has been affected.

Randomly mutagenizing both the strains will end up creating changes at random sequences in both the strains and this will tell us nothing about the gene that we are interested in.

RNAi refers to using a complementary RNA to the gene of interest. Since this gene is found in strain 1, using RNAi in strain 1 will result in the gene being silenced here and as a result the function that the gene was doing in strain 1 can be understood. Therefore, this method can be used to study the effect of the desired gene.

Fusing the gene with GFP in embryo will provide a method to track the expression of this gene as the embryo develops. It will help in localizing the protein product. However, this method in itself will give no information about the function of the gene.

A forward genetic screen refers to finding the genetic underlining for a given phenotype. This is now what is desired here.

Hence, the correct option is B.

gladiator answered 2 years ago

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