Plamitate uniformly labeled with tritium (3H) to a specific radioactivity of 3.0×·10^8 counts per minute per...

Plamitate uniformly labeled with tritium (3H) to a specific radioactivity of 3.0×·10^8 counts per minute per micromole was added to a mitochondrial preparation that oxidized 3H-palmitate to acetyl-CoA. The experiment calls for hydrolysis of the recovered acetyl-CoA to acetate and determination of the specific radioactivity of the acetate in counts per minute per micromole (cpm/μmol). Assuming that the palmitate is oxidized by the β-oxidation pathway and that there is no unlabeled fatty acid to dilute the tritium, what would be the expected specific radioactivity of the acetate? Show your reasoning and calculations.

Expert Solution

Plamitate uniformly labeled with tritium (3H) to a specific radioactivity of 3.0×·10^8 counts per minute per micromole was added to a mitochondrial preparation that oxidized 3H-palmitate to acetyl-CoA. The experiment calls for hydrolysis of the recovered acetyl-CoA to acetate and determination of the specific radioactivity of the acetate in counts per minute per micromole (cpm/μmol). Assuming that the palmitate is oxidized by the β-oxidation pathway and that there is no unlabeled fatty acid to dilute the tritium, what would be the expected specific radioactivity of the acetate? Show your reasoning and calculations.

queen_honey_blossom answered 1 year ago

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