Put the following events in the order in which you do them when performing P element...

Put the following events in the order in which you do them when performing P element transformation.

1. Cross injected flied individually with w/w flies of opposite sex

2. Clone a trans gene into a recombinant P element vector containing a white + marker gene

3. Cross red-eyed siblings to each other to generate transgenic lines, each with the recombinant P element in a different genomic location

4. Screen progeny of injected flies for red eyes

5. Inject w/w embryos with a mixture of two plasmids: a recombinant P element plasmid containing both your transgene and a white + marker gene, and a helper plasmid containing the P element transposes gene.

Expert Solution

Answer-

According to the given question-

P elements is also known as transposable elements found in Drosophila fly which work as main causative agents of genetic traits and these genetic traits also known as hybrid dysgenesis. This transposon are present in wild flies.

P element responsible for coding P transposase protein and the inhibitor of P transposase is expressed by wild type females and due to which the effect of P element as genome disruption is diminished and fertile progeny are produced.When we make a cross between the females which does not have P transposase inhibitor to the wild type males which posses P elements then we observe that due to lack of inhibitor for P transposase the P elements have the capability to proliferate in genome and causes disruption and causes death to progeny.

Steps-

3. Cross red-eyed siblings to each other to generate transgenic lines, each with the recombinant P element in a different genomic location

2. Clone a trans gene into a recombinant P element vector containing a white + marker gene

5. Inject w/w embryos with a mixture of two plasmids: a recombinant P element plasmid containing both your transgene and a white + marker gene, and a helper plasmid containing the P element transposes gene.

1. Cross injected flied individually with w/w flies of opposite sex

4. Screen progeny of injected flies for red eyes

gladiator answered 2 years ago

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