Which of the following steps would be necessary to determine the most effective antibiotic for a...

Which of the following steps would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessary/correct.

Collect a sample of bacteria from the infected patient.
Collect samples of bacteria from the bathroom taps in the patient’s room.
Collect samples of bacteria from other patients who seem to have the same infection.
Soak disks in many different types of antibiotic.
Soak disks in the antibiotic you have the biggest supply of.
Label the disks with the type of antibiotic you soaked them in.
Leave the petri dishes uncovered while the bacteria grow.
Cover the petri dishes while the bacteria grow.
Treat the bacterial culture with a disinfectant before spreading on the agar plate.
Wear personal protective equipment (gloves, mask, lab coat) while performing the procedure.
Wear gloves while performing the procedure.

Solutions

Expert Solution

Necessary steps:

1.- Collect a sample of bacteria from the infected patient.

This is important, because this is the only way we are going to obtain test results that will be useful for the specific patient, otherwise the results will be of no use

2.- Label the disks with the type of antibiotic you soaked them in.

Completely necessary to know which antibiotic is going to be discarded or used for prescription

3.- Cover the petri dishes while the bacteria grow.

In this way we protect the medium from getting contaminated

Unnecessary steps:

1.- Collect samples of bacteria from other patients who seem to have the same infection.

The susceptibility results obtained will be useful to treat the strains in the other patient, not in this original oen

2.- Leave the petri dishes uncovered while the bacteria grow.

Your medium will get contaminated and the results won't be useful

3.- Treat the bacterial culture with a disinfectant before spreading on the agar plate.

This will make impossible for the bacteria to grow on the medium, independently of their antibiotic susceptibility

gladiator answered 1 year ago

Next > < Previous

Related Solutions

[Auditing] 3. Which of the following controls would be most effective in detecting a misstatement of...

[Auditing] 3. Which of the following controls would be most effective in detecting a misstatement of the completeness assertion with respect to accounts payable? a. A list of outstanding checks written to vendors is generated during the preparation of the monthly bank reconciliation. b. Purchase orders must be accompanied by properly approved purchase requisitions before they are submitted to vendors. c. The AIS purchases and disbursements application will not prepare a check to a supplier without a valid ID number...

Explain the steps in writing an effective essay. Which, do you believe, is the most important?

Which internal control procedure would be most effective in dealing with the following threats? (a) Receiving-dock...

Which internal control procedure would be most effective in dealing with the following threats? (a) Receiving-dock personnel steal inventory and then claim the inventory was sent to the warehouse. (b) A company is late in paying a particular invoice. Consequently, a second invoice is sent, which crosses the first invoice’s payment in the mail. The second invoice is submitted for processing and also paid. (c) The petty cash custodian confesses to having “borrowed” $12,000 over the last five years. (d)...

Which of the following would likely be considered Congress's greatest (most effective, impacting and significant) power?...

Which of the following would likely be considered Congress's greatest (most effective, impacting and significant) power? A. Its power to coin money. B. Its power to establish immigration laws C. Its power to set up post offices D. Its "power of the purse"

Predict which of the following would be the most effective anti-freeze, assuming equal weight of each...

Predict which of the following would be the most effective anti-freeze, assuming equal weight of each are used: methyl alcohol(CH3OH) ; sucrose(C12H22O11); ethyl alcohol (C2H5OH); ethylene glycol ( C2H4(OH)2). Explain your choise!

Which of bacterial antibiotic resistance mechanisms most accurately describes this following scenario?

Which of bacterial antibiotic resistance mechanisms most accurately describes this following scenario? Scenario: MRSA has attained a new penicilin binding protein which inhibits beta-lactams from binding. This was probably obtained through _______ and is an example of __________ mechanism of reisistance: 1) point mutation, target mimicracy 2) transformation, target modification 3) conjugation, target overproduction 4)) transduction, drug inactivation

What antibiotic regime would be effective in treating H. Pylori if it were discovered as the...

What antibiotic regime would be effective in treating H. Pylori if it were discovered as the cause for this patients ulcer?

33. Which of the following would be most effective in a small owner/manager-operated business? a.cost centers...

33. Which of the following would be most effective in a small owner/manager-operated business? a.cost centers b.investment centers c.profit centers d.centralization 30. The following data relate to direct labor costs for March: Rate: standard, $12.00; actual, $12.25 Hours: standard, 18,500; actual, 17,955 Units of production: 9,450 The direct labor time variance is a.$2,362.50 unfavorable b.$6,540.00 unfavorable c.$2,362.50 favorable d.$6,540.00 favorable 29. The following data relate to direct materials costs for February: Materials cost per yard: standard, $1.94; actual, $2.05 Yards...

Which internal control procedure would be most cost-effective in dealing with the following expenditure cycle threats?...

Which internal control procedure would be most cost-effective in dealing with the following expenditure cycle threats? A purchasing agent orders materials from a supplier that he partially owns. Receiving-dock personnel steal inventory and then claim the inventory was sent to the warehouse. An unordered supply of laser printer paper delivered to the office is accepted and paid for because the “price is right.” After all of the laser printers are jammed, however, it becomes obvious that the “bargain” paper is...

Describe the steps that would be necessary to gain access to a formal organization for the...

Describe the steps that would be necessary to gain access to a formal organization for the purposes of doing field research. (Do not just list the steps, you need to describe what each is and what it entails)

Subjects