Question

Given the following stocks or chemicals in your stock room:

1) 3 L of 100 mM Tris-HCl pH 8.0
2) 100 mL of 0.5 M EDTA
3) 1 kg of NaCl (MW = 58.443g/mol)

4) 400 ml of 100% Ficoll 400

5) 100 g of Bromophenol Blue (MW= 669.96 g/mol)

Make the following solutions:
1) 20 ml of   6X Gel Loading Dye [5 % (v/v) Ficoll 400; 10 mM EDTA; 4 mM Tris-HCl pH 8.0; 10 mM NaCl; 15 mg/ml Bromophenol Blue]

Answer 1

The total amount of the solution is not given hence I have made the solution for 100ml.