Question

In: Biology

Blood typing_ how do you find out to which blood group someone belong? This is experiment...

Blood typing_ how do you find out to which blood group someone belong?
This is experiment in immunology lab
I want method, results and discussion
Of detect ( A and B) blood

Solutions

Expert Solution

To determine the blood-type you need anti-A and anti-B antibodies.

method

Make three tubes

  1. Contains mixture of (sample blood + anti-A antibodies)
  2. Contains mixture of (sample blood + anti-B antibodies)
  3. Only sample blood (negative control)

results

if there is agglutination seen in any of the first two or both, it suggests the positive results for respective antigen (A or B)

If a person has blood type A, the agglutination will only be senn in tube A, because only anti-A antibodies can act on antigen A present on RBCs of blood-type A.

If a person has blood type B, the agglutination will only be seen in tube B, because only anti-B antibodies can act on antigen B present on RBCs of blood-type B.

If a person has blood-type AB, the agglutination will be seen in both tubes 1 and 2, because anti-A and anti-B antibodies will act on antigen A and B present on RBCs of blood-type AB.

If a person has blood-type O, the agglutination will not be seen in any of the tubes because RBCs of blood-type O do not contain any of these two antigens.


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