Question

In capillary gel electrochromatography. a) what is the source of solute movement through the capillary? b) what drawbacks does this technique have? c) what are the advantages of this technique?

Please provide reference

Answer 1

a.

As name indicates its capillary action that responsible for solute movement.

To introduce the sample, the capillary inlet is placed into a vial containing the sample. Sample is introduced into the capillary via capillary action, pressure, siphoning, or electrokinetically, and the capillary is then returned to the source vial. The migration of the analytes is initiated by an electric field that is applied between the source and destination vials and is supplied to the electrodes by the high-voltage power supply.

b.

Drawbacks of gel electrophoresis-

1.Electrophorresis Has Limited Sample Analysis.

2 Electrophoresis Measurements Are Not Precise.

3 Substantial Starting Sample is Required.

Gel electrophoresis requires a large amount of DNA or protein to start with.

4 Only Certain Molecules Can Be Visualized.

Electrophoresis is excellent at separating and identifying medium- to large-sized biomolecules. However, many of the molecules that researchers wish to look at are smaller; small hormones, neurotransmitters, and ions cannot be measured by electrophoresis.

5 Electrophoresis is Low Throughput.

Gel electrophoresis is generally low throughput, meaning it doesn't produce data especially rapidly.

C.

1. Gel electrophoresis has some advantages.

2 Provides a clear link between similar results.

3 Relatively simple to perform.

4 Can test DNA from any type of evidence (hair, blood, skin, etc.)

5 Relatively inexpensive