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MICROBIOLOGY: CREATE a dichotomus key for the ALLL OF THE THINGS below! each test/microorganism must appear...

MICROBIOLOGY: CREATE a dichotomus key for the ALLL OF THE THINGS below! each test/microorganism must appear at least one:

Tests:

1. EMB 2. MSA 3. Blood Agar 4. PEA 5. SIM 6.Citrate 7.Phenol red (both glucose and sucrose) 8. BEA 9. Catalase. 10. Gram Stain

Bacterias:

1. Escherichia Coli

2. Enterobacteria Aerogene

3. Proteus Vulgaris
4. Serratia Marcescens

5. Pseudomonas Aerugenosa

6. Citrobacter Freundii

7. Staphylococcus Aureus

8. Staphylococcus Epidermidis

9. Entrococcus Faecalis

10. Mictococcus Leutues

Solutions

Expert Solution

  1. EMB- Escherichia Coli & Enterobacteria Aerogene because Eosin methylene blue is a selective stain for gram-negative bacteria
  1. MSA- Staphylococcus Aureus & Staphylococcus Epidermidis because Mannitol salt agar or MSA is used selective and differential growth medium
  1. Blood Agar - Staphylococcus Aureus because contains general nutrients and 5% sheep blood
  1. PEA- Staphylococcus Aureus Because a Gram positive organism, grows on this PEA plate
  1. SIM- Escherichia Coli Enterobacteria Aerogene, Proteus Vulgaris & Citrobacter Freundii
  1. Citrate - Enterobacteria Aerogene, Proteus Vulgaris & Citrobacter Freundii Because Citrate is an organic molecule that can be utilized by bacteria that produce the enzyme citrase
  1. Phenol red (both glucose and sucrose) - Staphylococcus Aureus & Staphylococcus EpidermidisBecause Staphylococcus aureus ferments mannitol S. aureus changes the color of the medium from pink to yellow due to acid by-products of mannitol fermentation
  1. BEA- Entrococcus Faecalis & Enterobacteria Aerogene, e.coli Because all are gram negative
  1. Catalase. - Entrococcus Faecalis Mictococcus Leutues & Staphylococcus Epidermidis Because all are gram positive
  1. Gram Stain- Staphylococcus Aureus & ECOLI

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