Question

1. 1. In polarizing microscopy, what is and what happens when a sample is anisotropic?

2. 2.What can happen to image of an anisotropic sample when you rotate it?

3.      3.What additional piece of equipment is added to a polarizing microscope to control anisotropic behavior? How does it accomplish control?

Answer 1

1. In polarizing microscopy, what is and what happens when a sample is anisotropic?

Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light. Simple techniques include illumination of the sample with polarized light. Directly transmitted light can, optionally, be blocked with a polariser orientated at 90 degrees to the illumination. More complex microscopy techniques which take advantage of polarized light include differential interference contrast microscopy and interference reflection microscopy.

These illumination techniques are most commonly used on birefringent samples where the polarized light interacts strongly with the sample and so generating contrast with the background. Polarized light microscopy is used extensively in optical mineralogy. Polarized light microscopy is capable of providing information on absorption color and optical path boundaries between minerals of differing refractive indices, in a manner similar to brightfield illumination, but the technique can also distinguish between isotropic and anisotropic substances. Furthermore, the contrast-enhancing technique exploits the optical properties specific to anisotropy and reveals detailed information concerning the structure and composition of materials that are invaluable for identification and diagnostic purposes

2.What can happen to image of an anisotropic sample when you rotate it?

In the field of computer graphics, an anisotropic surface will change in appearance as it is rotated about its geometric normal, as is the case with velvet.

Anisotropic filtering (AF) is a method of enhancing the image quality of textures on surfaces that are far away and steeply angled with respect to the point of view. Older techniques, such as bilinear and trilinear filtering, do not take into account the angle a surface is viewed from, which can result in aliasing or blurring of textures. By reducing detail in one direction more than another, these effects can be reduced.

3.What additional piece of equipment is added to a polarizing microscope to control anisotropic behavior? How does it accomplish control

The unique trapping properties arise because of the following factors. A linearly polarized beam propagating in the anisotropic fluid