What nucleotide does the branch point nucleotide bind in order to form the lariat intermediate? What...

What nucleotide does the branch point nucleotide bind in order to form the lariat intermediate? What is unusual about the bond between these two nucleotides?

Expert Solution

What nucleotide does the branch point nucleotide bind in order to form the lariat intermediate? What is unusual about the bond between these two nucleotides?

Brach point sequences are located on the upstream of 3′ end of an intron and the size varies from18 to 40 nucleotides. Though it is loosely consrved, adenine always remains. Spliceosome is responsible for the unusual 2′,5′-phosphodiester linkages in RNA lariats. The unusual linkage has to be hydrolyzed by the enzyme called intron debranching enzyme (Dbr1).This step is a rate limiting step and required for the process and efficient maturation of essential cellular factors such as small nucleolar RNAs (snoRNA) and micro RNAs (mirtrons). Spliceosomal splicing is a two step sequential transesterification reactions:

Lariat intermediate formed by the nucleophilic attack initiated by 2'OH of a specific branchpoint nucleotide within the intron on the first nucleotide of the intron at the 5' splice site.
Electrophilic attack at last nucleotide of the intron at the 3' splice site which is followed by first nucleotide due to 3'OH of released 5' exon and thus releases the lariant intron while joining exons.

gladiator answered 2 years ago

What is the main function of the legislative branch? What role does the executive branch play...

What is the main function of the legislative branch? What role does the executive branch play in the formation of laws? Summarize how these two branches work together.

what part of the cell membrane does HIV bind to

for cyanide what does it bind to/where does it act, explain what effect (if any) will...

for cyanide what does it bind to/where does it act, explain what effect (if any) will it have on "flow" or e-down the ETC, explain any impact on the apparent consumption of oxygen, explain any impact on the PMF and explain any impact on ATP production.

how does manganese bind to proteins? does manganese bind to other biopolymers? if so, how?

1. to which protein does calcium bind in order to expose the active sites 2. list...

1. to which protein does calcium bind in order to expose the active sites 2. list the four connective tissue structure associated with skeletal muscle

CO2 is a byproduct of cellular respiration. When does it form? a. intermediate step b. citric...

CO2 is a byproduct of cellular respiration. When does it form? a. intermediate step b. citric acid cycle c. glycolysis d. a & b e. all of these Which of these are involved during the direct synthesis of a protein? a. mRNA, small ribosomal subunit, amino acid b. tRNA, large ribosomal subunit c. RNA polymerase, transcription factors d. a and b e. all of these The deletion of a single base pair in the DNA can result in a. a...

How does Fluorouracil work, biologically - What enzyme/protein does this drug bind to? - What is...

How does Fluorouracil work, biologically - What enzyme/protein does this drug bind to? - What is the function of this enzyme/protein? - Where is its active site? - What is the substrate for the protein/enzyme? - What is the Km and Vmax of this enzyme if this information is available?

For Both DNP and Oligomycin - what does it bind to/where at, explain what effect if...

For Both DNP and Oligomycin - what does it bind to/where at, explain what effect if any it will have on the "flow" of e-down the ETC, explain any impact on the apparent consumption of oxygen, explain any impact on the PMF and explain an impact on the ATP production?

Describes the steps of translation initiation: What is the first tRNA to bind and where does...

Describes the steps of translation initiation: What is the first tRNA to bind and where does it bind? What is the role of IF3? Now describe elongation in prokaryotes - what occupies each of the A, P and E sites during elongation. What are the roles of Ef-Tu and Ef-G? Where is energy spent in the elongation process?

How does temperature and pH affect the ability of an enzyme to bind substrate? and what...

How does temperature and pH affect the ability of an enzyme to bind substrate? and what specific changes occur to the structure of an enzyme when it is boiled?

Question