Question

Trichinella sp. a) how to isolate (sputum, blood, fecal matter, etc.) b) where found-niche (water, air, food, etc.) c) what media (nutrient, starch, etc) d) selective growth condition (aerobic, anaerobic, etc.) e) identifying traits f) give virulent facts (those that help it be pathogenic) g) disease it may cause h) how each disease could be treated

Answer 1

a)

• The presence of Trichinella sp. in the feces , sputum blood etc can be identified by serological tests and other microscopic methods.
• The efficient way of detecting the sp is by using polymerse chain reaction.

b)

• It is transffered to humans from uncooked meat and other raw foods.
• It dugs deep into the mucosal layer of the intestine and remains there.
• It remains in the intracellular niches in both muscle and gastrointestinal infection.

c)

• Minimal essential medium with Earle's salts.
• Herk's balanced salt solution and minimal medium .
• L-glutamine should be added to both medium.
• The above 2 media can be used to grow the sp.

d)

• Trichinella sp are allowed to grow in anerobic and microaerophilic atmosphere.

e)

• Presence of heat shock protein in host and antibodies specific for trichinella species in the blood of humans and animals.

f)

• Virilent factors like nurse cell are produced by trichinella .
• This protects the developing larvae from host immune system.

g)

• It causes Trichinellosis.

h)

• Albendazole, mebendazole and corticosteroids are used to treat Trichinellosis.