In: Chemistry
What is the difference between a fluorescence excited spectrum and a fluorescence emission spectrum? Which one resembles the absorption spectrum? & Make a sketch showing absorptioina and fluorescence spectrum.
First of all, many compounds do not emit light after absorption
of radiation. so these molecules do not have an excitation
spectrum. The reason is that the absorbed energy can dissipate in
the molecule without emitting light/fotons.
Secondly, molecules absorb light to different excited states.
Emission comes from the lowest of these states, called the S1
state. When a molecule is excited to a higher state it often ends
up in this lowest excited state S1 and then emits radiation. In
this case the excitation spectrum is the same as the absorption
spectrum. However from a higher excited state a molecule does not
have to go to the lowest excited state. It can directly go to the
ground state for instance. Then it does not emit radiation. In this
case the excitation spectrum only has the lowest energy absoption
features. In spectroscopy terms: the absorption spectrum shows the
So-S1, the So-S2, So-S3 etc bands, but the excitation spectrum only
shows the So-S1 band.