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1. Next generation sequencing (NGS) has been instrumental in our every-expanding pursuit of genomic knowledge. reverse...

1. Next generation sequencing (NGS) has been instrumental in our every-expanding pursuit of genomic knowledge. reverse transcriptase is an enzyme uses mRNA as a template and in the presence of dNTP's makes a compliments DNA (cDNA, still technically not a violation of the central dogma

A. if i engrafted all RNA's from a cell then purified the spliced and poly-adenylated mRNA, could i use reverse transcriptase and illumine sequencing. Explain your answer

B. If I wanted to compare this sample to another sample (say diseased vs normal) explain how indeces help with this process. Also explain this potential approach over qRT-PCr

Please answer A and B

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gladiator answered 2 years ago
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