What are two of the distinguishing aspects of next generation sequencing (NGS) compared to earlier types...

What are two of the distinguishing aspects of next generation sequencing (NGS) compared to earlier types of sequencing?

Explain an advantage and a disadvantage to using next generation sequencing.

Expert Solution

The critical difference between treditional earlier sequencing and NGS is sequencing volume of gene. While the treditional earlier sequencing method only sequences a single DNA fragment at a time.

NGS is massively parallel, sequencing millions of fragments of DNA simultaneously per run. This high-throughput sequencing process translates into sequencing hundreds to thousands of genes at one time.

NGS also offers greater discovery power to detect novel or rare variants with deep sequencing of gene.

Advantages of Next generation sequencing:

Higher sensitivity to detect the low-frequency variants
Faster turn around time for sample volume is high
Comprehensive genomic coverage
Lower limit of the detection
Higher throughput with sample multiplexing
Ability to sequence range from hundreds to thousands of genes.

Disadvantages

Cost of the equipment is very high
Maintenence cost also high
There may be a chances of errors in data.

gladiator answered 1 month ago

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