Question

In: Biology

Learning Task 12-01 The Enzyme Lactase Experiment Design Check off the items in this list that...

Learning Task 12-01

The Enzyme Lactase Experiment Design

Check off the items in this list that you would use for this experiment. You may add any items you feel you would need.

  • Skim milk (which contains lactose)
  • Lactase drops
  • Graduated cylinder
  • Eye dropper
  • Ice cubes
  • pH paper
  • Hot plate
  • Mixing spoon
  • Thermometer
  • Vinegar (acidic)
  • Test tubes
  • Glucose test strips
  • Baking soda (basic)

Other items you feel you would need to use for this experiment.

Describe what you would do in your experiment. For each step, explain why the step is important. Add more steps if required.

Step:

Why this step is important:

If you actually conducted this experiment, what safety issues would you need to identify?

If you actually conducted this experiment, what appropriate precautions would you take to ensure that you performed the experiment safely?

If you actually conducted this experiment, what results would you expect to see?

Why would you expect these results?

Solutions

Expert Solution

The following are the materials to be required for the experiment-

a) Skim milk which contains lactose

b) Lactase drops

c) Test tubes

d) Glucose test strips

e) eye dropper

f) mixing spoon

g) graduated cylinder

Principle-

Lactase is an enzyme which catalyzes the hydrolysis of the disaccharide lactose into the monosaccharides galactose and glucose. Humans require lactase for digestion of lactose found in milk and other dairy products.

In this experiment, we will monitor the activity of the enzyme lactase by determining how much glucose is formed because glucose is a product of lactose hydrolysis by the enzyme lactase. We will monitor the enzyme activity using test strips that measure the level of glucose in the reaction mixture. The presence of glucose in solution will change the test strip from yellow to a blue-green color.

Method-

1) Prepare the following reaction in three test tubes-

A) Test tube with skim milk and enzyme solution (lactase). This is the test solution.

B) Test tube with skim milk and distilled water. This serves as negative control as this would not produce glucose

C) Test tube with glucose. This serves as positive control.

2) Time for 2 minutes and test for glucose with the glucose test strip.

Test tube A- strip will be Blue green color as lactase converts lactose into glucose and galactose

Test tube B- strip will be yellow colour as no glucose is formed due to the absence of the enzyme lactase.

Test tube C- strip will be blue green colour as there is glucose as a control in it.


Related Solutions

Learning Task 12-01 The Enzyme Lactase Experiment Design Which question will your experiment investigate? (select one...
Learning Task 12-01 The Enzyme Lactase Experiment Design Which question will your experiment investigate? (select one and delete the others) How does temperature affect how lactase drops work to break down lactose into glucose and galactose? How does pH affect how lactase drops work to break down lactose into glucose and galactose? How does agitation (movement) affect how lactase drops work to break down lactose into glucose and galactose? What variable will you change in this experiment? This is also...
The Enzyme Lactase experiment design Material required: Skim milk, lactase drops, test tubes, glucose test strips,...
The Enzyme Lactase experiment design Material required: Skim milk, lactase drops, test tubes, glucose test strips, eye dropper, mixing spoon, graduated cylinder Step: Why this step is important: If you actually conducted this experiment, what safety issues would you need to identify? If you actually conducted this experiment, what appropriate precautions would you take to ensure that you performed the experiment safely? If you actually conducted this experiment, what results would you expect to see?
The Enzyme Lab Design a controlled experiment to test the effect of salt concentration?
The Enzyme Lab Design a controlled experiment to test the effect of salt concentration?
Instrumental Learning- Design an experiment to test how modifying the differentiability of a behavior for in...
Instrumental Learning- Design an experiment to test how modifying the differentiability of a behavior for in fixed and variable interval schedules can change how resistant each schedule is to extinction.
Propose a new 'enzyme exercise' which could be adapted to online learning (i.e, perhaps an experiment...
Propose a new 'enzyme exercise' which could be adapted to online learning (i.e, perhaps an experiment that can be conducted in the kitchen). The experiment teaches the main concepts about enzymes and you describe an experiment with independent and dependent variables and controls. Feel free to submit something you found online and suggest modifications. Make sure to reference your sources. You may use your lab manual as a reference.
You are given task to design an experiment to convert 1 kg of water in ice...
You are given task to design an experiment to convert 1 kg of water in ice phase at 253 K and 100 kPa into vapor instantaneously. 1.  List down possible assumptions (with justification) to ease your experimental setup idea. 2. Sketch a schematic diagram of your experimental setup 3. Construct one experiment method (with step by step methodology) based on the collected diagram and selected apparatus and items used.
8. Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where...
8. Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?
Design and implement a program in python that takes a list of items along with quantities...
Design and implement a program in python that takes a list of items along with quantities or weights. The program should include at least two function definition that is called within the main part of your program. Each item has a price associated by quantity or weight. The user enters the item along with the quantity or weight and the program prints out a table for each item along with the quantity/weight and total price. Your program should be able...
Design an experiment to determine the amount of charge on a balloon. List the equipments, procedures,...
Design an experiment to determine the amount of charge on a balloon. List the equipments, procedures, and equations that would be used.
programming in python Design a program that initializes a list of 5 items to zero (use...
programming in python Design a program that initializes a list of 5 items to zero (use repetition operator). It then updates that list with a series of 5 random numbers. The program should find and display the following data: -The lowest number in the list - Average of the numbers stored in the list
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT