Can you hypothesize why a mixture of poly U+poly A would not stimulate incorporation of 14C-phenylalanine into protein?

Can you hypothesize why a mixture of poly U+poly A would not stimulate incorporation of ¹⁴C-phenylalanine into protein? (Refer to the table below)

Expert Solution

1.

RNA may form base pairing, during its folding or forming hybrid with complementary strands of DNA or RNA.
RNA have same purine bases as DNA: Adenine (represented as A) and Guanine (represented as G). RNA has Uracil (U) and Cytosine (C) as pyrimidine bases.
Thus, poly U will form complementary base pairing with poly A and will be blocked or unexposed for incorporation of phenyl alanine t-rna to bind to mRNA.

2.

During translation, tRNA carries amino acid to the ribosome, by matching anticodons to codons in mRNA.
The secondary structure of tRNA form the clover leaf structure with the anticodon arm having complementary nucleotides for base pairing with codons in mRNA.

Because poly u is complementary to poly A, Double stranded structures will be formed.

In order for an RNA to serve as a messenger RNA, it must be Single stranded , thereby exposing the bases for interaction with ribosomal subunits and tRNAs.

gladiator answered 2 years ago

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