Question

Using beers law find the amount of pigment isolated in grams for beta carotene. The e is 139500m^-1*cm^-1 and mw is 536.88 g/mol and the final weight is 3.08ml. The chlorophyll a has an e of 32580 and mw of 893.48g/mol and final volume is 3.22 ml.

The wavelength of beta carotene was found to be .544 at 450 nanometers and the chlorophyll a was .280 at 660nm not sure if this information is needed.

Answer 1

Although the question is a little doubtful, I'll try to answer it to the best of my knowledge.

According to Beer Lambert's law:

where A is the absorbance, is the molar extinction coefficient, c is the concentration and l is the path lengthof the sample cuvette. (Since you have not provided any information about pathlength, I'm taking it as 1cm because most of the spectrophotometers have 1cm sample cuvettes. In case it's different, please modify calculations accordingly)

1) Beta Carotene

(It's not wavelength. instead .544 is the absorbance at 450 nm, here 450 nm is the wavelength)

Now, conc. in M is given as -

Since we know molarity, mol. mass and volume, we can find the amount (in grams) of B-carotene as-

(M is nothing but mol/L)

2) Chlorophyll

Following the similar analogy, the concentration of chlorophyll is

So,