Question

How do you identify all of the genes in young leaves of Nicotiana tabacum when they are subjected to bugs. what are the molecular methods, and how and why are they used?

Answer 1

The linkage map consisting of 24 linkage groups has been constructed using simple sequence repeat (SSR) markers in Nicotiana tabacum.

Different method by which we identify the genes of young leaves of nicotiana tabacum are :-

1) Genome and root and leaf transcriptome sequencing :-10 mg of genomic DNA was broken into fragments of ~2–5?kb using the Covaris E220 Focused-ultrasonicators (LGC Genomics, Berlin, Germany) and purified on a 0.7% agarose gel to recover 3 kb and 5 kb fragments. After ends-repair, a Fasteris-designed spacer was ligated and the fragments were circularized.

2 ) Repeat content estimation:- The repeat content of the genome assemblies was estimated using RepeatMasker with the eudicot repeat library available from the Sol Genomics Network, the TIGR Solanaceae repeat library and RepeatScout59 libraries created using sequences of at least 200?kb from the draft genome assemblies.

3 ) Transcriptom assembly and quantitative analysis :-

The transcriptomes were derived by mapping RNA-Seq reads from each variety to the corresponding reference genome.