Question

1. What is the difference between a serine protease and a thiol protease? 2. How does cAMP differ from AMP? 3. What is the difference between a serine kinase, threonine kinase and a tyrosine kinase? 4. How are covalent modifications to monosaccharides made?

Answer 1

1. serine protease is an enzyme which cleaves the peptide bonds in protiens. here serine acts as nucleophillic amino acid at the active site of the enzyme.

thiol protease is an enzyme that degrades protiens. also called as cysteine proteases. These proteases have a common catalytic mechanism which involves nucleophilic cysteine thiol in the catalytic.

2. cAMP: It acts as a messenger in biological processes. it is a derivative of ATP. it is used for intracellular transduction.

AMP: It is a nucleotide. it consits of ribose sugar, phosphate group, and base adenine.it is importtant component RNA synthesis and plays an important role in cellular metabolism. it is interconverted to ADP or ATP.

3. serine kinase : it phosphorylates the OH group of serine.

threonine kinase : it phosphorylates the oh group of threonine.

receptors of the serine/ threonine kinase are useful in the regulation of programmed cell death, cell proliferation, cell differentiation, and embryonic development.

tyrosine kinase: it is an enzyme which transfers phosphate group from ATP to protein. it acts on or off switch in cellular mechanisms.

4. Covalent modifications to the monosaccharides is made by combining hydroxyl group of a monosaccharide with the hydrogen of the other monosaccheride. it is also called as glycosidic linkage.