why is pUC19 plasmid that make it useful as a vector for ligations and transformations

Expert Solution

puc 19 plasmid is more frequently used in bactrial transformation reaction to carry the desired gene.

The features which make it useful to use in transformation experiment are

--Selectable marker- its has ampicillin resistant gene. By using this marker we can easily differentiate between the E coli cell which containing the plasmid or not.

E COLI cell with out plasmid- not grow in amp media

E coli cell with plasmid- grow on ampicillin media

- it has Multiple cloning site ( MCS) for insertion of desired gene. As this MCS has multiple enzyme site so we can select any RE site dep6upon our requirement

--- it has beta galactosidase gene under Lac Z promoter

Bu using this enzyme, by using single step methods we can easily recognize the colony which has plamid containing desired gene known as blue white selection.

e coli cell with plasmid containing desired gene- white colony

E coli cell , having plasmid without desired gene - blue colony

So based on these features it is very easy to use the plasmid in the transformation experiment.

gladiator answered 2 years ago

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