Introducing a plasmid or vector into a bacterium such as E. coli is called_____________. a. recombination ...

Introducing a plasmid or vector into a bacterium such as E. coli is called_____________.

a. recombination b. transfection c. transformation

d. ligation e. replication

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Expert Solution

Ans- c) transformation is the process by which plasmid or vectors are introduced in a bacterial cell. After digestion with restriction enzymes and ligation of plasmid with DNA to be be introduced into the cell Transformation occurs. After its uptake cloning of this recombinant molecules occur in bacteria.

a) Recombination - it refers to the process when two DNA molecules exchange their genetic information. The two DNA molecules are broken by endonucleases and further joined by ligases to produce new combination of alleles.

b) Transfection - the process of introducing oforeign DNA molecules into the nucleus of Animals by using chemical ,physical or biological method is known as transfection.

d) Ligation - the process of formation of phosphodiester bond between two DNA molecules by using ligase enzyme is known as ligation.

e) Replication - replication simply means making copies of the genetic material inside cell.

gladiator answered 1 year ago

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