Introducing a plasmid or vector into a bacterium such as E. coli is called_____________. a. recombination ...

Introducing a plasmid or vector into a bacterium such as E. coli is called_____________.

a. recombination b. transfection c. transformation

d. ligation e. replication

Expert Solution

Ans- c) transformation is the process by which plasmid or vectors are introduced in a bacterial cell. After digestion with restriction enzymes and ligation of plasmid with DNA to be be introduced into the cell Transformation occurs. After its uptake cloning of this recombinant molecules occur in bacteria.

a) Recombination - it refers to the process when two DNA molecules exchange their genetic information. The two DNA molecules are broken by endonucleases and further joined by ligases to produce new combination of alleles.

b) Transfection - the process of introducing oforeign DNA molecules into the nucleus of Animals by using chemical ,physical or biological method is known as transfection.

d) Ligation - the process of formation of phosphodiester bond between two DNA molecules by using ligase enzyme is known as ligation.

e) Replication - replication simply means making copies of the genetic material inside cell.

gladiator answered 2 years ago

The bacterium Escherichia coli (or E. coli) is a single-celledorganism that lives in the gut...

The bacterium Escherichia coli (or E. coli) is a single-celled organism that lives in the gut of healthy humans and animals. When grown in a uniform medium rich in salts and amino acids, these bacteria swim along zig-zag paths at a constant speed of 20 μm/s. The figure shows the trajectory of an E. coli as it moves from point A to point E. Each segment of the motion can be identified by two letters, such as segment BC. For the...

Your lab is studying gene expression of the tryptophan operon in the bacterium E. coli. In...

Your lab is studying gene expression of the tryptophan operon in the bacterium E. coli. In order to facilitate this study, you have cloned the Trp-operon, excised the five coding genes (TrpA, B, C, D, and E), and replaced them with a copy of the reporter gene GFP (green fluorescent protein). The resulting recombinant DNA was inserted into an F' plasmid, and used to transform a number of different strains of E. coli. For each of the scenarios listed below,...

A student is examining a bacterium under the microscope. The E. coli bacterial cell has a...

A student is examining a bacterium under the microscope. The E. coli bacterial cell has a mass of m = 1.80 fg (where a femtogram, fg, is 10−15g) and is swimming at a velocity of v = 2.00 μm/s , with an uncertainty in the velocity of 5.00 % . E. coli bacterial cells are around 1 μm ( 10−6 m) in length. The student is supposed to observe the bacterium and make a drawing. However, the student, having just...

“In the model organism E. coli, recombination mediated by the related XerC and XerD recombinases complexed...

“In the model organism E. coli, recombination mediated by the related XerC and XerD recombinases complexed with the FtsK translocase at specialized dif sites, resolves dimeric chromosomes into free monomers to allow efficient chromosome segregation at cell division. Computational genome analysis of Helicobacter pylori, a slow growing gastric pathogen, identified just one chromosomal xer gene (xerH) and its cognate dif site (difH). Here we show that recombination between directly repeated difH sites requires XerH, FtsK but not XerT, the TnPZ...

Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...

Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment, plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 5) What does LB (-) plate contain? 6) What does LB/ amp (-) plate contain? 7) What does LB/amp (+) plate contain? 8) What does LB/amp/ara (+) plate contain?

A pathogenic (disease-causing) E. coli bacterium living on a cold piece of dirty romaine lettuce in...

A pathogenic (disease-causing) E. coli bacterium living on a cold piece of dirty romaine lettuce in your fridge suddenly finds itself in the nice, warm, and cozy human intestine. How will this bacterium maintain the same level of membrane fluidity now that it's in a such a different environment? produce membrane lipids with hydrocarbon tails that are longer and have more double bonds produce membrane lipids with hydrocarbon tails that are longer and have fewer double bonds produce membrane lipids...

Assume you have purified the insert-containing plasmid from chloramphenicol or kanamycin-resistant E. coli. You used pBLU...

Assume you have purified the insert-containing plasmid from chloramphenicol or kanamycin-resistant E. coli. You used pBLU as the vector for the unknown insert project. You use PCR to identify your unknown insert. What is your positive control, negative control, and experimental group?

Experiment 3: Cloning a DNA Fragment into a Bacterially-Derived Plasmid Vector Foregin DNA = 720 Plasmid...

Experiment 3: Cloning a DNA Fragment into a Bacterially-Derived Plasmid Vector Foregin DNA = 720 Plasmid DNA 2804 Post-Lab Questions 1. What is the expected size of the plasmid plus the cut foreign DNA? 2524 base pairs 2. What type of ends do the enzymes BamHI and EcoRI produce? How does this type of end facilitate cloning? 3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA molecule? How does...

why is pUC19 plasmid that make it useful as a vector for ligations and transformations

Which of the following statements is TRUE? E. coli sigma factors all recognize one E. coli...

Which of the following statements is TRUE? E. coli sigma factors all recognize one E. coli gene Prokaryotic mRNAs contain sequences for sigma factor binding mRNA in both E. coli and humans are translated with the same translation machinery E. coli sigma factors all bind to the core RNA polymerase mRNA always contains the nucleotide sequence encoding just one protein

Question