How is DNA fragmented with restriction enzymes? What sites are recognized? How do we determine how...

How is DNA fragmented with restriction enzymes? What sites are recognized? How do we determine how often recognition sites are found? Please provide an explanation.

Expert Solution

A restriction enzyme (or restrictase) cleaves DNA into fragments at or near specific recognition sites within DNA known as restriction sites and the enzymes make two incisions through each sugar-phosphate backbone of the DNA double helix.

gladiator answered 2 years ago

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