There are hundreds of different restriction enzymes, each of which cuts a specific target DNA sequence....

There are hundreds of different restriction enzymes, each of which cuts a specific target DNA sequence.

a. What are the two types of cuts different restriction enzymes can make?

b. In a cloning procedure, why would you use the same restriction enzyme to excise the target DNA sequence and to cut the plasmid? (1 mark)

c. What enzyme would you use for the next step and what would it do?

Expert Solution

gladiator answered 1 year ago

A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts...

A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts at position 100. HindIII cuts at positions 500 and 2000. Which of the following represents the fragment that would be observed if the restricted DNA were analyzed using agarose gel electrophoresis? A. 400, 100, 1000, 1500 B. 400, 500, 1500 C. 400, 100, 1600 D. 500, 100, 500, 900 E. 500, 1000, 600

Describe what a palindromic sequence is in DNA. How do restriction enzymes work? What would the...

Describe what a palindromic sequence is in DNA. How do restriction enzymes work? What would the sticky ends be for EcoR1 (which cuts GAATTC between the G and A)?

explain the role of that DNA ligase, restriction enzymes and plasmids have in creating recombination DNA

The unpaired nucleotides/single-stranded DNA produced by the action of restriction enzymes on plasmid DNA are referred...

The unpaired nucleotides/single-stranded DNA produced by the action of restriction enzymes on plasmid DNA are referred to as? a. sticky ends b.blunt ends c. triple Strand DNA d.ligases

What are restriction enzymes, and why are they used in recombinant DNA technology? (3 marks)

What are restriction enzymes, and why are they used in recombinant DNA technology?

The enzyme required for transcription is * RNA polymerase Restriction enzymes Splicesome DNA polymerase

Imagine you have a plasmid and you cut it with three different restriction enzymes (enzymes 1,...

Imagine you have a plasmid and you cut it with three different restriction enzymes (enzymes 1, 2, 3). You then run these fragments through a gel and get this separation. Label the positive and negative terminals on this gel with a (+) and (-) sign. If you were working with a single plasmid, how many cut sites did restriction enzyme 1 have? Explain? When creating a gel, we stain it with Ethidium Bromide or Gel Red. What is the purpose...

Outline the roles played by restriction enzymes and vectors in cloning DNA. Words to choose from:...

Outline the roles played by restriction enzymes and vectors in cloning DNA. Words to choose from: bacterial, eukaryotic, nucleotide, DNA, RNA, protein, palindrome, nuclease, protease, phage, plasmid. 1. A restriction enzyme is a _________ ____________that recognizes specific _________ sequences in a ________molecule, often a __________, and cleaves or nicks the molecule at those sites. 2. A vector is an agent into which a foreign DNA segment is inserted and used to transform host cells. A vector can be created...

. For each of the restriction enzymes listed below: (i) Approximately how many restriction fragments would...

. For each of the restriction enzymes listed below: (i) Approximately how many restriction fragments would result from digestion of the human genome (3 x 109 bases) with the enzyme? (ii) State whether the fragments produced by digestion with each enzyme would have sticky ends with a 5’ overhang, sticky ends with a 3’ overhang, or blunt ends. (The recognition sequence for each enzyme is given in parentheses, where N means any of the four nucleotides. ^ marks the site...

Background Calculating Transformation Efficiency Restriction digest & ligation: You add restriction enzymes to 0.05ug of each...

Background Calculating Transformation Efficiency Restriction digest & ligation: You add restriction enzymes to 0.05ug of each plasmid in separate pAMP and pKAN tubes tubes and heat the restriction enzymes by placing them in a hot water bath for 20 minutes. Afterwards, you label 1 tube pAMP/pKAN and combine 4 ul of digested pAMP, 4 ul of digested pKAN, 1 ul of ligase, and 1 ul of ligase buffer and leave it to incubate overnight. You then label an empty tube...

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