Question

1.The purpose of the indole test is to differentiate groups of gram-negative, lactose-fermenting bacteria called___________ . This specific test allows to identify bacteria that are capable of hydrolyzing the amino acid___________ . This is achieved by detecting the presence of the enzyme___________

2.Which of the following products are made from this hydrolysis reaction? (select all)

You can select more than one option

A.Indole

B.Oxygen

C.Ammonia

D.Pyruvate

E. Glucose

F.Carbon Dixoide

G. Citrate

Glucose

F

Carbon dioxide

Answer 1

1..The purpose of the indole test is to differentiate groups of gram-negative, lactose-fermenting bacteria called Klebsiella. his specific test allows to identify bacteria that are capable of hydrolyzing the amino acid Tryptophan.This is achieved by detecting the presence of the enzyme tryptophanase.

2.

• Ammonia - the enzyme urease (EC 3.5. 1.5) catalyzes a reaction in which one molecule of urea is hydrolyzed to form two molecules of ammonia (NH₃) and one carbonic acid (H₂CO₃) via the formation of carbamic acid (H₃CNO₂)
• Glucose - The digestion of carbohydrates by enzyme catalysed hydrolysis begins in your mouth and continues in your stomach and small intestine. The final product of the digestion of carbohydrates are monosaccharides such as glucose and fructose.
• Citrate - The citric acid cycle starts with a condensation reaction of the four-carbon oxaloacetate with the two-carbon acetyl group of acetyl CoA. Oxaloacetate reacts with acetyl CoA and water to give citrate and CoA as products. This reaction, an aldol condensation followed by a hydrolysis, is catalyzed by citrate synthase.

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