In: Biology
1. Consider the problem of comb shape inheritance in chickens, where walnut, rose, pea, and single ae observed as distinct phenotypes. How is comb shape inherited, and what are the genotypes of the P1 generation of each cross. Use the following data to answer these questions. Test the results in cross 4 to your hypothesis. (30 pts.) Cross 1: single X single all single Cross 2: walnut X walnut all walnut Cross 3: rose X pea all walnut Cross 4: F1 X F1 of Cross 3 walnut X walnut 98 walnut 28 rose 34 pea 9 single
F. Write out the genotypes of the four crosses. Do they make sense in terms of the offspring phenotypes and the hypothesis that you made in E? G. How do you test your hypothesis from the information in Cross 4? H. Execute the test. How do you get the expected numbers for each class? I. How many degrees of freedom are there? What is the cutoff value for significance? J. How do you interpret the result?
Null hypothesis -
Comb shape in poultry is inherited as a non mendelian character. It is an epistatic phenomena. Epistasis is a non mendelian inheritance in which alleles at one gene locus mask the effect of alleles at other gene locus.
Phenotype | Genotype |
A_bb | rose |
aaB_ | pea |
aabb | single |
A_B_ | walnut |
1. aabb × aabb = aabb (single)
2. AaBB × AABb = A_B_ (walnut)
3. AAbb × aaBB = AaBb (walnut)
4. AaBb × AaBb =
A_B_ = walnut = 98
A_bb = rose = 28
aaB_ = pea = 34
aabb = single = 9
# progenies = 98 + 28 + 34 + 9 = 169
Test for cross 4 -
Phenotype | #observed | #expected | (O-E)^2/E |
Walnut | 98 | 9/16 × 169 = 95.06 | 0.0909 |
Pea | 34 | 3/16 × 169 = 31.68 | 0.128 |
Rose | 28 | 3/16 × 169 = 31.68 | 0.427 |
Single | 9 | 1/16 × 169 = 10.56 | 0.23 |
Calculated chi square value = 0.0909 + 0.128 + 0.427 + 0.23 = 0.8759
Degrees of freedom = 4 - 1 = 3
P value = 0.9 to 0.95
Interpretation : null hypothesis accepted
Comb shape in poultry is inherited as an epistatic character.
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