Question

 

 

1) What are some examples of chromatography and how is the technique used in the biochemistry laboratory?

 

2) What are some features of disaccharides that helps distinguish a complete abbreviation of a compound?

Answer 1

Examples of chromatography

1)ion exchange chromatography 2)resin chromatography 3)paper chromatography 4)It helps in checking the level of pesticides, fungicides, and contaminants in the food and drinking water 5)Percentage purity is also found by chromatography 6)Creating vaccinations 7)Beverage testing 8) Drug testing 9)Forensic testing

Use in biochemistry: It is widely used in biochemical research for the separation and identification of chemical compounds of biological origin. In the petroleum industry, the technique is employed to analyze complex mixtures of hydrocarbons. This is also used in the purification of proteins bound to tags

A disaccharide is the sugar formed when two monosaccharides (simple sugars) are joined by glycosidic linkage. When disaccharides like sucrose are digested, they are broken down into their simple sugars and used for energy.As in all glycosides, the glycosidic bond does not allow mutarotation. Since this type of bond is formed stereospecifically by enzymes in natural disaccharides, they are only found in one of the possible configurations (α or β).

The human ABO blood groups illustrate the effects of glycosyl- transferases. Carbohydrates are attached to glycoproteins and glycolipids on the surfaces of red blood cells. For one type of blood group, one of the three different structures, termed A, B, and O, may be present (Figure given below). These structures have in common an oligosaccharide foundation called the O (or sometimes H) antigen. The A and B antigens differ from the O antigen by the addition of one extra monosaccharide, either N-acetylgalactosamine (for A) or galactose (for B) through an α-1,3 linkage to a galactose moiety of the O antigen.