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1. The restriction enzyme SauIIIA recognizes the 4 bp sequence GATC, and the "6-cutter" enzyme BamHI...

1. The restriction enzyme SauIIIA recognizes the 4 bp sequence GATC, and the "6-cutter" enzyme BamHI recognizes the sequence GGATCC. Digestion of a particular bacteriophage genome produced a total of 160 SauIIIA fragments. Approximately how many BamHI fragments would you expect if:
A) the phage genome has a “G+C” content of 50%?
B) the phage genome is 80% G+C?

2. At least some of the RNA sequences below include significant regions that are self-complementary, and therefore might form secondary structures such as hairpins or stem-loops. For purposes of this problem, you should identify which sequences have any secondary structures that fit the following criteria:

a minimum length of 5 base pairs


only “Watson/Crick” pairings (i.e., A-U or G-C), with NO mismatches or unpaired bases within the helical/paired region


connecting "hairpin" turns or loops that consist of at least 3 unpaired bases.


(Note that these conditions do not always apply to the secondary structures formed by naturally occurring RNA molecules.)

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gladiator answered 2 years ago
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