Question

Compare and contrast SYBR Green versus TaqMan?

Answer 1

SYBR green:

• It is an asymmetrical cyanine dye used as nucleic acid strain in molecular biology.

• It binds to Dna forms the complex that is best absorbs at 497nm at blue light and produces(emits) green light.

• It preferably binds to double strand dna but can also strain Rna with lower performance than ssDNA(single strand).

• Used in several fields of biochemistry and molecular biochemistry.

• Used as dye for dna quantification in quantitative PCR (polymerized chain reaction).

• Used in labelling pure nucleic acid strain in dna in flow cytometry ad fluorescence microscopy.

Taqman:

• Its a hydrolysis probe to increase the specificity of quantitative PCR.

• It relies on the principle of 5' - 3' exonuclease activity of taq-polymerase to cleave a duel labelled probe during hybridization to target sequence and fluorescence based detection.

• Intrestingly the name originated of taqman is from video game that is taq-polymerase + pacman= taqman.

• The fluorescence detected in the quantitative PCR thermal cycler is directly proportional to the fluorophore released and the amount of DNA template present in the PCR.

• Its widely used in quantitative PCR in research and medical research like

• Pharmacogenomics, gene expression assays, HLA- genotyping,  

• Dna quantification assays, bacterial identification assays, verification of microbiology results etc.