A presentation on maltose standard curve

Expert Solution

The standard curve for maltose is usually consstructed using 3,5-Dinitro salicylic acid (DNS) as the reagent.

Maltose reduces the pale yellow colores alkaline DNS to the orange-red colored, 3 amino,5 nitro salicylic acid.

The intensity of the color is proportional to the concentration of maltose present in the solution. This intensity change in color is measured using a calorimeter as the and the absorbance is at 540 nm wavelength. This wavelenght is selected because, it is the region where the orange-red color absorbs.

A series of solutions containing varying concentrations of maltose are prepared in test tubes and a known quantity of DNS is added to each. These test tubes are heated on a water bath for few minutes and their optical densities are measured using a colorimeter.

A graph is then plotted with amount of maltose on X axis and the observed optical density at Y axis. The plot obtained is the standard maltose curve.

gladiator answered 3 months ago

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