Question

In: Biology

Briefly speculate how complexation with certain PEGylated polycationic polymers such as PLL-PEG can increase the potency...

Briefly speculate how complexation with certain PEGylated polycationic polymers such as PLL-PEG can increase the potency of hydrophobically modified RNAi molecules.

Solutions

Expert Solution

The presence of positive charges on the surface of PLL/siRNA complexes is believed to enhance cell internalization. However, interaction of the positively charged PLL/siRNA complexes with negatively charged serum proteins may cause undesired aggregation and RES uptake, which consequently decrease the therapeutic outcome of siRNA.

Modification of PLL with polysaccharides, PEG, or other water-soluble polymers is the major strategy to enhance the systemic circulation profile of PLL/siRNA complexes by decreasing nonspecific interaction with blood components.

PEGylation of PLL leads to formation of particles with a core-shell structure. Such particles contain a cationic fragment in the inner core that helps to condense nucleic acids. The uncharged hydrophilic PEG outer layer helps to reduce particle size, cytotoxicity, and nonspecific interaction with blood components, leading to prolonged systemic circulation.


Related Solutions

An experiment was conducted to observe the effect of an increase in temperature on the potency...
An experiment was conducted to observe the effect of an increase in temperature on the potency of an antibiotic. Three 1-ounce portions of the antibiotic were stored for equal lengths of time at each of the following Fahrenheit temperatures: 40, 55, 70, and 90. The potency readings observed at the end of the experimental period were Potency reading, y: 49 38 27 24 38 33 19 28 16 18 23 Temperature, x: 40 40 40 55 55 55 70 70...
An experiment was conducted to observe the effect of an increase in temperature on the potency...
An experiment was conducted to observe the effect of an increase in temperature on the potency of an antibiotic. Three 1-ounce portions of the antibiotic were stored for equal lengths of time at each of the following Fahrenheit temperatures: 40, 55, 70, and 90. The potency readings observed at the end of the experimental period were Potency reading, y: 49 38 27 24 38 33 19 28 16 18 23 Temperature, x: 40 40 40 55 55 55 70 70...
How is pain sensation modulated by other sensory input? Please briefly describe the mechanism and speculate...
How is pain sensation modulated by other sensory input? Please briefly describe the mechanism and speculate on why such a modulatory pathway evolved.
How is pain sensation modulated by other sensory input? Please briefly describe the mechanism and speculate...
How is pain sensation modulated by other sensory input? Please briefly describe the mechanism and speculate on why such a modulatory pathway evolved.
How is pain sensation modulated by other sensory input? Please briefly describe the mechanism and speculate...
How is pain sensation modulated by other sensory input? Please briefly describe the mechanism and speculate on why such a modulatory pathway evolved. Please leave a detailed description. Thank you :)
Appraise the adjustable peg, crawling peg and managed floating exchange rate systems and elaborate how the...
Appraise the adjustable peg, crawling peg and managed floating exchange rate systems and elaborate how the Monetary Authority of Singapore (MAS) conducts its monetary-exchange rate policy (a diagram is useful).
You would like to speculate on a rise in the price of a certain stock. The...
You would like to speculate on a rise in the price of a certain stock. The current stock price is RM25, and a three-month call option with a strike price of RM30 costs RM2.5. You have RM1,000 to invest. Identify two alternative strategies, one involving an investment in stock and the other involving investment in option. What are the potential gain and losses from each?
You would like to speculate on a rise in the price of a certain stock. The...
You would like to speculate on a rise in the price of a certain stock. The current stock price is $40 and a three-month call with a strike price of $42 costs $2.50. You have $5,000 to invest. Identify two alternative strategies. Outline the advantages and disadvantages of each.
Briefly explain how import tariffs can be part of a set of government policies to increase...
Briefly explain how import tariffs can be part of a set of government policies to increase national well-being for small developing countries where government revenue is difficult to obtain by other means such as an income or sales tax.
For a telecommunication company ( Jio telecommunication company as example) Speculate how benchmarks can be determined...
For a telecommunication company ( Jio telecommunication company as example) Speculate how benchmarks can be determined when evaluating variances from standard costs. Provide detailed examples and how you arrived at those examples.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT