In: Biology
In the drawings below note and label all important elements (incl. consensus sequences) discussed in lectures and tutorial manual and listed below.
a) Prokaryotic operon: promoter (-10 and -35 elements), operator, multiple structural genes (for example 3), start site of transcription, start sites of translations, transcription termination sequence
b) Prokaryotic mRNA (polycistronic): transcription start site, multiple ribosome binding sites (i.e. Shine-Dalgarno sequence in E. coli), multiple ORFs (including start and stop codons), transcription termination sequence
c)Eukaryotic gene: promoter (TATA box), consensus sequence CAAT, enhancer, transcription start site, exons, introns, translation start site (i.e. start codon), stop codon, polyadenylation sequence
d) Eukaryotic mRNA: transcription start site, ORF (incl. start and stop codon), polyadenylation sequence, poly A tail, 5’ methyl cap, 5’ and 3’ untranslated regions. (0.25 marks for each label; up to 7 marks)