Question

(4) Name four (4) enzymes/proteins that play a role in human DNA replication.

Answer 1

Enzymes/Proteins that play role in Human DNA replication:

1. Helicase:

The parenteral DNA has Its own helicase. It is essential in DNA replication which separates double-stranded parental DNA into single strands. Then it continues to unwind the DNA and forms two replication pork. For the process of breaking of hydrogen bonds between the nucleotide, it requires energy. Helicase uses the energy stored in the ATP.

2. Single-Stranded DNA Binding Protein:

SSB protein binds with high affinity to the single-stranded portion of DNA. DNA polymerases access their substrate by destabilizes the helical duplexes. SSB has recombination in vivo in the DNA replication. It combines with the purine and pyrimidine to form base-pair with nucleotides.

3. DNA polymerase III:

It performs the polymerization of nucleotides. It adds to a new nucleotide begins in the 3' end of a strand and extends to 5 form a 5' to 3' direction. the newly formed strand is complementary to the parental strand. Then it synthesizes with an RNA primer to initiate DNA synthesis.

4. DNA polymerase I:

The main function is to remove the RNA primers after the DNA replication. It removes the ribonucleotides from the end of the RNA primer 5 in the form of 5' to 3' direction. Also, the exonuclease fills the gap of the necessary nucleotide with the neighboring Okazaki fragment.