An enzyme was obtained in Dr. Cai’s lab. To study the
kinetics of this enzyme, a
graduate student measured the velocity of the reaction
at a substrate concentration
of 0.2 mM substrate. He found the initial rate decreased
to 1/3 after 1.2 mM
competitive inhibitor was added.
KI, the dissociation constant for
the inhibitor
binding to the enzyme, is 0.5 mM. What is the
KM, the Michaelis
constant?
A. 0.33 mM
B. 0.5 mM
C. 0.1 mM
D. 1 mM...