In: Chemistry
For each of the following categories of compounds, indicate the type of liquid chromatography that could be used to separate analytes in that category. (Drag the appropriate chromatography label to the blank next to the category. Only one label applies to each category, but a label may be used more than once).
Seperation Mode Analyte Category
Fm>2000,soluble in octane,size10 nm
Fm>2000, soluble in butanol, size 100 nm
Fm>2000, soluble in water,nonionic,size 10 nm
Fm<2000, soluble in in ethanol
Fm<2000 , soluble in water, ion-paired
Fm<2000, soluble in water, ionic
Choices:
ion -exchange
Bonded-reverse phase
Adsorption
Bonded normal phase
Molecular exculsion
Ans: For 1,3 it is reverse phase due to
1) Fm>2000,soluble in octane,size10 nm : Bonded-reverse phase
2) Fm>2000, soluble in butanol, size 100 nm : Molecular exculsion
3) Fm>2000, soluble in water,nonionic,size 10 nm : Bonded-reverse phase
4) Fm<2000, soluble in in ethanol : Bonded normal phase
5) Fm<2000 , soluble in water, ion-pair : Bonded normal phase
6) Fm<2000, soluble in water, ionic : ion -exchange
For 1,3 it is reverse phase due to the presence of non polar in nature, where as for no.2 we have to use Molecular exculsion because of more size
For 4 and 5 it is bonded normal phase due to the presence of polar solvents, where as for no.6 it is ion -exchange because of ionic in nature.