For each of the following categories of compounds, indicate the type of liquid chromatography that could...

For each of the following categories of compounds, indicate the type of liquid chromatography that could be used to separate analytes in that category. (Drag the appropriate chromatography label to the blank next to the category. Only one label applies to each category, but a label may be used more than once).

Seperation Mode Analyte Category

Fm>2000,soluble in octane,size10 nm

Fm>2000, soluble in butanol, size 100 nm

Fm>2000, soluble in water,nonionic,size 10 nm

Fm<2000, soluble in in ethanol

Fm<2000 , soluble in water, ion-paired

Fm<2000, soluble in water, ionic

Choices:

ion -exchange

Bonded-reverse phase

Adsorption

Bonded normal phase

Molecular exculsion

Solutions

Expert Solution

Ans: For 1,3 it is reverse phase due to

1) Fm>2000,soluble in octane,size10 nm : Bonded-reverse phase

2) Fm>2000, soluble in butanol, size 100 nm : Molecular exculsion

3) Fm>2000, soluble in water,nonionic,size 10 nm : Bonded-reverse phase

4) Fm<2000, soluble in in ethanol : Bonded normal phase

5) Fm<2000 , soluble in water, ion-pair : Bonded normal phase

6) Fm<2000, soluble in water, ionic : ion -exchange

For 1,3 it is reverse phase due to the presence of non polar in nature, where as for no.2 we have to use Molecular exculsion because of more size

For 4 and 5 it is bonded normal phase due to the presence of polar solvents, where as for no.6 it is ion -exchange because of ionic in nature.

queen_honey_blossom answered 1 month ago

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