Question

T/F (TRUE/FALSE)

ASAP PLEASEE ANSWER ALL

1.UV spectroscopy is an example of non-radiative decay. Visible spectroscopy relies on radiative decay. This is why we can see colors

2.P-value tells you if a data point is statistically insignificant and can be discarded from a data set.

3.Standard deviation and confidence interval both relate to the scatter around a mean.

4.In fluorescence, emitted photons are released in all directions. Typically they are only detected at a right angle to the irradiating beam of light.

5.Electroporation is a method to introduce plasmids into bacterial cells.

6,Isothermal Titration Calorimetry (ITC) provides access to complete thermodynamic parameters (∆H, ∆S, ∆G) as well as K­­d and binding stoichiometry.

7.Electroporation is a method to introduce plasmids into bacterial cells.

Answer 1

1. FALSE, It utilizes absorption or reflection, not non-radioactive decay in this spectroscopy. Use of electrons involved in the chemical reaction

2. TRUE. P value shows statistical significance of the data, if P value is more than 0.05 then it considered as insignificant and can be discarded in majority of instances (P>0.05 not significant).

3 FALSE. Standard deviation is the difference in the data set and confidence interval depict the accuracy of the average of data and it is related to standard error (which can be calculated from SD)

4. TRUE. Absorbed at 90 degree (right angle) due to high absorption of wavelength at this direction

5. TRUE, Due to electric current pore of bacterial cell open and plasmid can easily pass through in transformation, very common technique in microbiology

6. TRUE, ITC determine the thermodynamic approaches, this parameter is suitable for drugs specially to determine the interaction with biomolecules like DNA and proteins.

7. TRUE (as mentioned in 5), repeated question