Question

ŕeview the polymeerase chain reaction pcr and arrange the sentences into a logical paragraph

Answer 1

Polymerase Chain Reaction.
PCR is a technique which is used to form copies of a specific region of DNA. The main aim of PCR is to amplify that particular region of the DNA so that it can be analyzed for further studies. The DNA polymerase used in this process is Taq polymerase isolated from a heat tolerant bacterium Thermus aquaticus. So the polymerase works fine at around 0 degree celcius at which the two strands of DNA are separated. The PCR primers are short 20 bp long primers which attaches to the two opposite strans of the DNA at the particular region of choice. When the primers bind to the DNA templates, the polymerase extends them forming copies of the region of choices. The basic steps of PCR includes Denaturation, Annealing and Extension. Denaturation is carried out at 96 degree celsius the DNA strands are heated to separate them for the availability of single stranded DNA strand for the next step. In annealing step, the reaction is cooled up to 55-65 degree celsius so that the primer can bind to the single stranded DNA. The final step is extension which is performed at 72 degree celsius, at which, the Taq polymerase extends the DNA strands. This cycle is carried on for 25-30 cycles. The final result of PCR is visualized with the help of Gel Electrophoresis.