Question

In: Statistics and Probability

1. most appropriate statistical tst 2. null 3. Alternative hypothesis 4. Calculate appropriate test statistic 5....

1. most appropriate statistical tst 2. null 3. Alternative hypothesis 4. Calculate appropriate test statistic 5. A statistical conclusion

The following are results of a protein standard curve assay for the presence of a protein. A standard protein of known concentration was added to each tube and the OD600 was measured on a spectrophotometer. Determine protein conc in unknown sample with a OD600 of 0.345.

Protein Conc (ug/ul) OD600
0 0.000
10 0.081
20 0.118
30 0.203
60 0.378
90 0.626
120 0.777
180 1.088

Solutions

Expert Solution

1) The most appropriate statistical test is Wilcoxon Sign rank test,since the sample is less than 30 we go for non parametric test for testing mean.

2) H0: OD600 , = 0.345  

3) H1 : OD600 , 0.345.

4)

Test Statistic: 56.5

5) Statistical Conclusion:

At 5% level of significance,we reject H0 ,since(p value=0.01166 < 0.05).therefore,we conclude that protein conc in unknown sample is not with a OD600 of 0.345. OD600 , 0.345.

orchestra answered 3 years ago
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