In: Statistics and Probability
1. most appropriate statistical tst 2. null 3. Alternative hypothesis 4. Calculate appropriate test statistic 5. A statistical conclusion
The following are results of a protein standard curve assay for the presence of a protein. A standard protein of known concentration was added to each tube and the OD600 was measured on a spectrophotometer. Determine protein conc in unknown sample with a OD600 of 0.345.
Protein Conc (ug/ul) | OD600 |
0 | 0.000 |
10 | 0.081 |
20 | 0.118 |
30 | 0.203 |
60 | 0.378 |
90 | 0.626 |
120 | 0.777 |
180 | 1.088 |
1) The most appropriate statistical test is Wilcoxon Sign rank test,since the sample is less than 30 we go for non parametric test for testing mean.
2) H0: OD600 , = 0.345
3) H1 : OD600 , 0.345.
4)
Test Statistic: 56.5
5) Statistical Conclusion:
At 5% level of significance,we reject H0 ,since(p value=0.01166 < 0.05).therefore,we conclude that protein conc in unknown sample is not with a OD600 of 0.345. OD600 , 0.345.