I
have a question about qPCR.
How to measure an mRNA level of a gene by qPCR. If I perform a
realtive quantification qPCR, first I need to convert mRNA into DNA
template by Reverse transcriptase. then I perform a qPCR reaction.
For example, comparing with the reference gene, the drug treatment
lead to a decrease in the expression of gene of interest. However,
I think that can only determine the gene replication is inhibited
because the pcr reaction is...