Question

In: Biology

Describe/diagram two different types of RNAP III promoters. Diagram/describe an RNAP II promoter including the startpoint...

Describe/diagram two different types of RNAP III promoters.

Diagram/describe an RNAP II promoter including the startpoint for the RNA transcript.

(If you could answer just briefly that would be great)

Solutions

Expert Solution

RNAP III,also known as RNA Polymerase III are multisubunits(17 subunits) enymes that transcribe tRNAs and 5S rRNA in eukaryotes. The genes transcribed by them are known as "House Keeping" genes. Structure of RNAP III promoters is very complex:

  1. Type 1 promoter is recognized by gene specific transcription factors TFIIIA, TFIIIC (bind with recognition site) TFIIIB-? (bind with promoter site), and RNAP III itself at the 5s gene.
  2. Type 2 promoter consists of TFIIIC, TFIIIB-? and RNAP III at the tRNA genes.

RNAP II, also known as RNA Polymerase II is also a multisubunits enzyme and transcribed m - RNA. RNAP II promoter has four elements: Upstream element, TATA Box, Initiator, and Downstream element. Transcription factor TFIID bind at TATA Box, TBP also bind at TATA Box, then bind TFIIA with TFIID and TFIIB at the Initiator region then finally bind the RNAP II along withTFIIF attach with this complex and special trancription factor interact with upstream element. So, this special trancription has two domains, one is DNA bind domain and the other is polymerase binding domain.


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