A microscope is used to examine a sample of bacteria that are spread on glass. If...

A microscope is used to examine a sample of bacteria that are spread on glass. If the diameter of the objective is twice its focal length and the sample is near the focal point of the objective, determine the shortest distance between two bacteria that can be resolved under the microscope in the terms of the wavelength ? of the light source.
.

Solutions

Expert Solution

The shortest distance between two objects that can be resolved, when viewed with an optical instrument is known as resolution limit d of the instrument. For microscope it is given as,

Here, ? is the wavelength of the light used to illuminate the microscope

NA is numerical aperture for the objective lens and it is given as

Here, n is the refractive index of the medium in which objective lens is placed (For air n =1)

? is the angle of cone of the light coming from objective.

Given:

Diameter of the objective is twice the focal length,

Sample is near focal point

Solution:

From the figure above Numerical aperture can be given as,

For microscope resolution limit is given as,

Substituting the value of numerical aperture in above equation we have,

Thus, the shortest distance between two bacteria that can be resolved under the microscope in the terms of the wavelength ? of the light source is .

genius_generous answered 2 months ago

Next > < Previous

Related Solutions

1) When viewing a mixed culture of bacteria under a microscope, you notice that there is...

1) When viewing a mixed culture of bacteria under a microscope, you notice that there is significant diversity in their arrangements and shapes.Describe the types of bacterial cell arrangements and what determines the diversity in these arrangements.Explain why Bacilli are less varied in arrangement than cocci? (Minimum length : 150 words).

histology and microscopy, what are the steps required to view bacteria samples using a compound microscope?...

histology and microscopy, what are the steps required to view bacteria samples using a compound microscope? How to select the bacteria cultures for viewing? How to prepare the samples using the different slide preparation techniques? How to set up the compound microscope to ensure a good image quality is obtained?

1.Fused silica glass is used for expensive space vehicle windows , whereas sodalime glass is used...

1.Fused silica glass is used for expensive space vehicle windows , whereas sodalime glass is used for low cost glass windows for homes. Why is the cost of making these two glasses so much different. Answer this question by; a)comparing the specific processing conditions required for each b)comparing the specific properties which require these processing conditions c)comparing the specific microstructural features which result in these properties

Data from a sample of 10 pharmacies are used to examine the relationship between prescription sales...

Data from a sample of 10 pharmacies are used to examine the relationship between prescription sales volume and the percentage of prescription ingredients purchased directly from the supplier. The sample data is given below (Use formula to answer each question). percent ingredient 10 18 25 40 50 63 42 30 5 55 sales volume in $1000 25 55 50 75 110 138 90 60 10 100 a. Draw scatter plot between percent ingredient (x-variable) and the sale volume (y variable)....

You have reviewed bacteria and how they cause disease. In some cases the spread of bacterial...

You have reviewed bacteria and how they cause disease. In some cases the spread of bacterial infections is actually caused by human error. In the Microbial Pie case, you saw how improperly prepared food, poor hand washing, or improperly stored food can cause outbreaks of bacterial infections. In remote areas this could occur as well. Discuss what measures you would take in a remote area of the world where you may be sent by WHO. Remember, you have limited resources,...

A researcher is looking at a cellular sample using an electron microscope. He observes that while...

A researcher is looking at a cellular sample using an electron microscope. He observes that while mRNA is being transcribed from the DNA that ribosomes are binding to the mRNA to make proteins. Is this sample from a bacterial or a eukaryotic cell? Explain your answer to receive full credit.

You take a tissue sample from a wound. You look at it under the microscope and...

You take a tissue sample from a wound. You look at it under the microscope and see bacteria inside epidermal cells, surrounding the nucleus. Which of the following activities must have occurred to get the bacteria there? (Select all that apply) Group of answer choices Attachment Colonization Invasion Toxin production Replication Biofilm formation

1a. How is biological sample prepared for the insertion into the transmission electron microscope for single...

1a. How is biological sample prepared for the insertion into the transmission electron microscope for single particle averaging? b. The single image of a molecule in cryo-EM is very noisy. What is done to improve the clarity of the image? What does that mean in terms of statistics?

In a polarized optical microscope, under a crossed polarized system, a sample of an anisotropic crystal...

In a polarized optical microscope, under a crossed polarized system, a sample of an anisotropic crystal is placed, and the interference color will be changed if the state is rotated. At this time, if the polarizing plate is removed and the natural light is directly irradiated to the crystal, can the change of interference color also be observed when the stage is rotated? Why? Please give the explaination.

12) What limits the resolving power of a light microscope? A) The type of lens used...

12) What limits the resolving power of a light microscope? A) The type of lens used to magnify the object under study. B) The shortest wavelength of light used to illuminate the specimen. C) The type of lens that focuses a beam of electrons through the specimen. D) The type of heavy metal or dye that is used to stain the specimen. 13) The advantage of light microscopy over electron microscopy is that A) Light microscopy provides for higher magnification...

Subjects